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技術文章ARTICLE

人TNF-α,IL-1β,E2 ELISA試劑盒引用文獻

點擊次數(shù)：12673 發(fā)布時間：2022/3/4 14:14:57

【文獻標題】Dehydroepiandrosterone alleviates intestinal inflammatory damage via GPR30-mediated Nrf2 activation ａnd NLRP3 inflammasome inhibition in colitis mice

【作者】Ji Cao , Miaomiao Lu , Weiyuan Yan , et.al

【作者單位】南京農業(yè)大學（Nanjing Agricultural University）

【文獻中引用產品】

人腫瘤壞死因子α(TNF-α)ELISA試劑盒

人白細胞介素1β(IL-1β)ELISA試劑盒

人雌二醇(E2)ELISA試劑盒

【關鍵詞】Dehydroepiandrosterone ,GPR30 ,Nrf2 ,NLRP3 inflammasome ,Colitis

【影響因子(IF)】7.37

【出版期刊】《Free Radical Biology ａnd Medicine》

【產品原文引用】

Sigma (St Louis, MO, USA) provided the DHEA, dimethyl sulfoxide (DMSO) ａnd LPS (Escherichia coli 055:B5). The Opti-MEM medium ａnd fetal bovine serum (FBS) were obtained from Gibco (Erie, NY, USA). The dulbecco＇s modified Eagle＇s medium (DMEM) ａnd trypsin-EDTA were all obtained from Biological Industries (Kibbutz Beit-Haemek, Israel). Corning (NY, USA) provided the cell culture dishes.

TRIZOL Reagent Kits, SYBR Green Master Mix ａnd reverse transcriptase kit were all purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Jiancheng Bioengineering Institute of Nanjing (Nanjing, China) provided the Nitric Oxide (NO), malondialdehyde (MDA) ａnd total superoxide dismutase (SOD) detection kits. Shanghai hengyuan biological technology Co., Ltd. (Shanghai, China) provided the TNF-α, IL-1β ａnd Estradiol (E2) ELISA kits. Beyotime Biotechnology Institute (Shanghai, China) provided the glutathione peroxidase, nitric Oxide (NO), protein contents assay kits ａnd N-Acetyl-L-cysteine (NAC). MCE (St. Louis, MO, USA) provided the inhibitors including G1, G15, SB203580, U0126, BAY11-7082, GKT137831, TAK-242, 3-MA, Fulvestrant, Flutamide, ML385 ａnd RTA-408.